Crude protein content is the main indicator for imported food, feed, and oil, and the quality of measurement results directly affects the interests of both parties. With the ever-increasing imports of soybeans and barley in recent years, this influence has become increasingly important. The Kjeldahl method SN/T0803.9-1999 is the standard method for the determination of crude protein in imported and exported oils. The automatic Kjeldahl determination instrument is the main instrument for the determination. The laboratory tries to evaluate and calculate the uncertainty of the product. To quantitatively characterize the quality of measurement results and determine the credibility of the measurement results.

Accurately weigh a certain amount of soybean sample and prepare it according to the SN/T0800.1 standard. Decompose the protein with sulfuric acid in the presence of a catalyst. Measure the nitrogen content with an automatic Kjeldahl determination instrument and multiplied by the corresponding nitrogen and protein conversion factor. That is, the crude protein content. Uncertainty results from reading deviations and other contingent changes, including random effects of mass, random effects of volume, sample inhomogeneity, and random effects during testing. The uncertainty comes from the resolution of the balance, the indication tolerance and temperature error of the glass container, the calibration of the nitrogen determination system, the purity of chemical reagents, and the uncertainty of the atomic weight.

Through the determination of automatic Kjeldahl determination of nitrogen, the uncertainty evaluation method for the determination of crude protein content in soybeans was discussed. The r value of the interlaboratory collaboration test was used to assess and indirectly assess the system effect due to the recovery test. The hydrochloric acid standard solution titration volume uncertainty component, has strong practicality and operability, suitable for crude protein analysis of food, feed, oil.

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